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methods in enzymology volume iv【2025|PDF下载-Epub版本|mobi电子书|kindle百度云盘下载】

methods in enzymology volume iv
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图书目录

Section Ⅰ．Techniques for Characterization of Proteins (Procedures and Interpretations)1

1．Electrophoresis＆ARNE TISELIUS2

1A．Paper Electrophoresis＆A．HELGE F．LAURELL21

2．Ultracentrifugation，Diffusion，and Viscometry＆H．K．SCHACHMAN32

3．Infrared Spectrophotometry＆D．L．WOOD104

4．X-Ray Diffraction of Protein Crystals＆F．H．C．CRICK127

5．Light-Scattering Measurements＆M．BIER147

6．Flow Birefringence＆W．F．H．M．MOMMAERTS166

7．Fluorescence Techniques for the Enzymologist＆DONALD J．R．LAURENCE174

8．The Solubility Diagram as a Criterion of Protein Homogeneity＆ROGER M．HERRIOTT212

9．Determination of Amino Acid Sequence in Proteins＆R．R．PORTER221

10．The Pipsyl Method for Amino Acid Sequences in Proteins＆MILTON LEVY238

11．Methods for Investigating the Essential Groups for Enzyme Activity＆H．FRAENKEL-CONRAT247

Section Ⅱ．Techniques for Metabolic Studies271

12．Techniques for the Assay of the Respiratory Enzymes＆BRITTON CHANCE273

13．Use of Artificial Electron Acceptors in the Study of Dehydrogenases＆J．H．QUASTEL329

14．Study of soil Metabolism Using the Perfusion Technique＆J．H．QUASTEL and P．G．SCHOLEFIELD336

15．Methods for Study of the Hill Reaction＆WOLF VISHNIAC342

16．Methods for Measurement of Nitrogen Fixation＆R．H．BURRIS and P．W．WILSON355

17．Micromethods for the Assay of Enzymes＆O．H．LOWRY366

18．Histochemical Methods for Enzymes＆GEORGE GOMORI381

19．Electron Microscopy of Cellular Constituents＆FRITIOF S．SJOSTRAND391

Section Ⅲ．Techniques for Isotope Studies423

20．The Measurement of Radioisotopes＆DANIEL STEINBERG and SIDNEY UDENFRIEND425

21．The Measurement of Stable Isotopes＆ANTHONY SAN PIETRO473

22．The Synthesis and Degradation of Isotopically Labeled Carbohydrates and Carbohydrate Intermediates＆S．ABRAHAM and W．Z．HASSID489

23．Determination of Isotopic Carbon Patterns in Carbohydrate by Bacterial Fermentation＆I．A．BERNSTEIN and HARLAND G．WOOD561

24．Isotopic Experimentation with Intermediates of the Tricarboxylic Acid Cycle＆H．E．SWIM and M．F．UTTER584

25．Synthesis and Degradation of Isotopically Labeled Glyeolic，Glyoxylic，and Oxalic Acids＆KATHARINE F．LEWIS and SIDNEY WEINHOUSE609

26．Purines and Pyrimidines＆EDWARD D．KORN615

27．Biosynthesis of Protoporphyrin＆DAVID SHEMIN643

28．Methods for Chemical Synthesis，Isolation，and Degradation of Labeled Compounds as Applied in Metabolic Studies of Amino Acids and Proteins＆DAVID M．GREENBERG and MORTON ROTHSTEIN652

29．Synthesis and Degradation of Labeled Steroids＆K．BLOCH732

30．Labeled Compounds Related to Methyl Group Biosynthesis and Transfer＆J．A．STEKOL751

31．The Synthesis of Labeled Sulfur Compounds＆HAROLD TARVER769

32．The Synthesis and Degradation of Carbon-Labeled Fatty Acids and Related Compounds＆H．S．ANKER779

33．Labeled Compounds in the Study of Phospholipid Metabolism＆CAMILLO ARTOM809

34．Preparation and Analysis of Labeled Coenzymes＆SIDNEY P．COLOWICK and NATHAN O．KAPLAN840

35．Synthetic and Analytic Procedures Involving I131-Labeled Compounds＆ALVIN TAUROG and I．L．CHAIKOFF856

36．Intermediates of Photosynthesis:Isolation and Degradation Methods＆A．A．BENSON and M．CALVIN882

37．O18 Containing Phosphate and Related Compounds＆MILDRED COHN905

AUTHOR INDEX915

SUBJECT INDEX941

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